NEPA21高效基因轉(zhuǎn)染儀------適用于體外(In Vitro)和活體(In Vivo)類器官轉(zhuǎn)染,讓轉(zhuǎn)染更簡(jiǎn)單、更Free
NEPA21高效基因電轉(zhuǎn)染儀采用全新設(shè)計(jì)的電轉(zhuǎn)程序,專門(mén)針對(duì)難轉(zhuǎn)染細(xì)胞、微藻、受精卵、離體組織、動(dòng)物活體等的高效轉(zhuǎn)染利器,配合專利的電壓衰減(Voltage Decay)設(shè)計(jì),NEPA21可在獲得高轉(zhuǎn)染效率的同時(shí),提高細(xì)胞存活率。具有高轉(zhuǎn)染效率及高細(xì)胞存活率的優(yōu)點(diǎn)。
NEPA21電轉(zhuǎn)染儀主要特點(diǎn):
- 全新設(shè)計(jì)的電轉(zhuǎn)程序
- 特別適用于難轉(zhuǎn)染細(xì)胞、離體組織、動(dòng)物活體的轉(zhuǎn)染
- 適用于胚胎基因編輯
- 高轉(zhuǎn)染效率、高細(xì)胞存活率
- 電轉(zhuǎn)程序各項(xiàng)參數(shù)可見(jiàn)、可調(diào),適用性廣
- 不需要特殊的轉(zhuǎn)染試劑盒輔助,運(yùn)維成本低
NEPA21電轉(zhuǎn)染儀應(yīng)用范圍:
適用范圍:原代細(xì)胞、干細(xì)胞、以及各種難轉(zhuǎn)染細(xì)胞(如免疫細(xì)胞、血液細(xì)胞等)
適用范圍:可以直接對(duì)貼壁細(xì)胞進(jìn)行轉(zhuǎn)染,省去了細(xì)胞消化、再貼壁的步驟(對(duì)提高某些種類細(xì)胞的存活率十分重要)!
離體組織轉(zhuǎn)染
適用范圍:組織切片、腦切片、器官、胚胎等
適用范圍:大腦、視網(wǎng)膜、肌肉、皮膚、肝臟、腎臟、睪丸等
日本NEPA GENE公司專業(yè)研發(fā)、生產(chǎn)細(xì)胞電轉(zhuǎn)染儀、電融合儀及單細(xì)胞分離提取系統(tǒng)等,其生產(chǎn)NEPA21系列電轉(zhuǎn)染儀、ECFG21高效細(xì)胞電融合與電穿孔儀及PicoPipet單細(xì)胞分離提取系統(tǒng)在研究領(lǐng)域中久負(fù)盛名,已被數(shù)百篇文獻(xiàn)引用,其中不乏高水平雜志的文章,如Nature、Cell、PNAS、Genes & Dvelopment等。
- Hendriks D, Pagliaro A,
Andreatta F, et al. Human fetal brain self-organizes into long-term expanding
organoids[J]. Cell, 2024.
- Diaz-Cuadros M, Wagner D E,
Budjan C, et al. In vitro characterization of the human segmentation clock[J]. Nature, 2020, 580(7801): 113-118.
- Nakazawa Y, Hara Y, Oka Y, et
al. Ubiquitination of DNA damage-stalled RNAPII promotes transcription-coupled
repair[J]. Cell, 2020, 180(6):
1228-1244. e24.
- Barnabe-Heider et al. Genetic
manipulation of adult mouse neurogenic niches by in vivo electroporation. Nature Methods, 2008 Feb;5(2):189-96.
- Limura T and Pourquie O.
Collinear activation of Hoxb genes during gastrulation is linked to mesoderm
cell ingression. Nature, 2006 Aug
3;442(7102):568-71.
- Sanada K and Tsai LH. G
Protein betagamma Subunits and AGS3 Control Spindle Orientation and Asymmetric
Cell Fate of Cerebral Cortical Progenitors. Cell, 2005 Jul 15;122(1):119-31
- Ladher RK et al. FGF8
initiates inner ear induction in chick and mouse. Genes and Development, 2005 Mar 1;19(5):603-13.